Oxidative stress is involved in cancer development. Hydrogen (H2) is a potentantioxidant and exhibits anti-inflammatory and potentially anticancer-like activities.This study aimed to investigate the role of H2incombination with 5-fluorouracil(5-FU) in cancer treatment both in vitro and in vivo using the colon 26 cell line.
The survival rate was determined using the Kaplan–Meier survival test, and cellviability was assessed using cell viability imaging kit and the MTT assay, andactivation of the cell apoptosis pathway (Phosphorylated adenosine monophosphateactivated protein kinase (p-AMPK), Apoptosis-inducing factor (AIF) and Caspase3) were characterized by western blots.
Hydrogen water administration improvedthe survival of mice with colon 26-induced cancer. Furthermore, hydrogen waterenhanced cell apoptosis in cancer cells, resulting in a marked increase in theexpression of p-AMPK, AIF and Caspase 3 in colon 26 cells. Hydrogen water alsoincreased the inhibitory effect of 5-FU on colon 26 cells with spect to cell survivalrate and anticancer functions. Additionally, high-content hydrogen water exhibitedstronger antioxidative and anticancer activity than did the natural hydrogen water.In conclusion, high-content hydrogen water can inhibit colon cancer, particularly incombination with 5-fluorouracil.